The clinical studies** done for Ganoderma Reishi Mushroom Peptide focus on the efficacy in reducing wrinkles, smoothing skin, and reducing dark spots through both in vitro and in vivo experiments. Two main studies were conducted:

Study 1: In Vitro Gene Expression Study

This study assessed the gene expression changes in human dermal fibroblasts after 24-hour exposure to a 1% solution of used Ganoderma Peptide. The study revealed significant upregulation in several genes responsible for skin repair and anti-aging, indicating that the product promotes dermal repair and helps prevent oxidative and cellular damage. The results are summarized below:

Gene	Role	% Increase
P4HA1	Collagen formation	195%
PXN	Cell adhesion and migration	134%
LOXL2	Collagen crosslinking	105%
B4GALT7	Glycosaminoglycan biosynthesis	89%
CTGF	Tissue regeneration	71%

Study 2: In Vivo Clinical Study

This study involved 15 Caucasian female volunteers aged 45-65 with deep crow’s feet wrinkles and dark spots. The study used a placebo-controlled, half-face design where one side of the face was treated with a placebo cream and the other with 2% Ganoderma Peptide for 28 days. The following parameters were assessed:

• Wrinkle Reduction: Ganoderma Peptide significantly reduced the surface area and volume of crow’s feet wrinkles over 14 and 28 days compared to the placebo.

Parameter	% Change (Day 14)	% Change (Day 28)
Wrinkle Surface Area	-13%	-26%
Wrinkle Volume	-15%	-30%

• Skin Roughness: A decrease in roughness parameters (Ra, Rz, Rt) was observed, indicating smoother skin around the eye area.

Roughness Parameter	% Change (Day 14)	% Change (Day 28)
Ra (Average Roughness)	-6%	-11%
Rz (Wrinkle Depth)	-8%	-12%
Rt (Maximum Height)	-7%	-10%

• Dark Spot Reduction: Ganoderma Peptide significantly reduced melanin concentration on dark spots, leading to a more even skin complexion.

Melanin Concentration	% Change (Day 14)	% Change (Day 28)
Melanin Levels	-7.3%	-8.4%

• Subjective Evaluation: After 28 days, the majority of subjects reported visible improvements in wrinkle reduction, smoother skin, and a more even complexion, with over 80% of participants satisfied with the results.

**As per the data sheet supplied. Juhst has not conducted these clinical studies on the peptide.